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  • 无菌房间内部设备和用具操作及消毒
    来源: 浏览:次 发布日期:2020-09-15
    1.无菌房间内部的work台,不论是什么材质、用途的,都要旨表面光滑和台面水平。
    1. The worktable in aseptic room, no matter what material and use, requires smooth surface and horizontal table.
    2.在内室和外室各安装一个紫外灯(多为30W)。内室的紫外线灯应安装在经常work的座位正上方,离地上2m,外室的紫外线灯可安装在外室。
    2. Install an ultraviolet lamp (mostly 30W) in the inner and outer chambers. The ultraviolet lamp in the inner room should be installed directly above the working seat, 2m away from the ground, and the ultraviolet lamp in the outer room can be installed in the center of the outer room.
    3.外室应有专用的work服、鞋、帽、口罩、盛有来苏儿水的瓷盆和毛巾、手持喷雾器和5百分比石炭酸溶液等。
    3. the outer rooms should have special work clothes, shoes, caps, masks, pottery basins and towels containing the suer water, hand held sprayers and 5百分比 carbolic acid solutions.
    4.内室应有酒精灯、常用接种工具、不锈钢制的刀、剪、镊子、70百分比的酒精棉球、工业酒精、载玻璃片、特种蜡笔、记录本、铅笔、标签纸、胶水、废物筐等。
    4. There should be alcohol lamp, common inoculation tools, stainless steel knife, scissors, tweezers, 70百分比 alcohol cotton ball, industrial alcohol, glass carrier, special crayon, record book, pencil, label paper, glue, waste basket, etc.
    无菌室的灭菌消毒
    Sterilization of aseptic room
    1.薰蒸:这是无菌室彻底灭菌的措施。无菌室使用了较长时间,污染比较严重时,应进行薰蒸灭菌。可用甲醛、乳酸或硫磺薰蒸。
    1. Fumigation: This is a measure to completely sterilize the sterile room. If the aseptic room has been used for a long time and the pollution is serious, it should be fumigated and sterilized. It can be fumigated with formaldehyde, lactic acid or sulfur.
    实验室装潢
    2.喷雾:在每次使用无菌室上进行。喷雾可促使空气中微粒及微生物沉降,防止桌面、地上上的微尘飞场,并有杀菌感化。可用5百分比石炭酸喷雾。
    2. spray: before the use of asepsis room. Spray can cause particles and microorganisms to settle in the air, preventing dust flying on the desktop and ground, and having bactericidal effect. It can be sprayed with 5百分比 carbolic acid.
    3.紫外线照射:在每次使用无菌室上进行。紫外线有较好的杀菌成果。一贯应开启紫外线灯照射30~60min。
    3. Ultraviolet radiation: before each use of sterile room. UV has good germicidal efficacy. Generally, the ultraviolet lamp should be turned on to irradiate for 30-60min.
    无菌室work规程
    Working procedure of aseptic room
    1.无菌室灭菌。每次使用上开启紫外线灯照射30min如该,或在使用上30min,对内外室用5百分比石炭酸喷雾。
    1. Sterilization in aseptic room. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5百分比 carbolic acid spray in the interior and exterior chambers.
    2.用肥皂洗手下,把所需器材搬入外室;在外室换上已灭菌的work服、work帽和work鞋,戴好口罩,然下用2百分比煤酚皂液将手浸洗2分钟。
    2. After washing hands with soap, move the required equipment into the outer room; change into sterilized work clothes, work caps and work shoes in the outer room, wear masks, and then soak hands with 2百分比 coal phenol soap for 2 minutes.
    3.将各种需用物品搬进内室清点、就位,用5百分比石炭酸在work台面上方和操作员站位容量喷雾,返回外室,5~10min下再进内室work。
    3. move all necessary articles into the inner room for inventory and position. Spray the 5百分比 carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
    4.接种操作上,用70百分比酒精棉球擦手;进行无菌操作时,动作要轻缓,尽量减少空气波动和地上扬尘。
    4. Before inoculation, wipe hands with 70百分比 alcohol cotton ball; during aseptic operation, the action should be gentle to minimize air fluctuation and ground dust.
    5.work中应care安全。如遇棉塞着火,用手紧握或用湿布包裹熄灭,切勿用嘴吹,以免扩大燃烧;如遇有菌培养物洒落或打碎有菌容器时,应用浸润5百分比石炭酸的抹布包裹下,并用浸润5百分比石炭酸的抹布擦拭台面或地上,用酒精棉球擦手下再继续操作。
    5. Pay attention to safety in work. If the cotton plug is on fire, hold it tightly with your hands or wrap it with a wet cloth. Do not blow it with your mouth to avoid expanding combustion. In case of bacterial culture spilled or broken, wrap it with a cloth soaked in 5百分比 carbolic acid, and wipe the table or ground with a cloth soaked in 5百分比 carbolic acid, and then continue to operate after wiping hands with alcohol cotton ball.
    6.work结束,立即将台面收拾干净,将不应在无菌室存放的物品和废弃物全部拿出无菌室下,对无菌室用5百分比石炭酸喷雾,或开紫外线灯照射30min。
    6. at the end of the work, clean up the table immediately, and remove all the articles and wastes that should not be stored in the asepsis room, then use the 5百分比 carbolic acid spray or the ultraviolet lamp to irradiate 30min.
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