1、 Decoration standard of PCR Laboratory (data required for PCR laboratory construction)
Gene amplification laboratory decoration design is a professional design and decoration project (PCR laboratory planning and Design). The design of gene amplification laboratory usually has four zones: reagent storage and preparation area, specimen preparation area, accounting amplification area and product analysis area. These four zones do not need to be connected together, and they must be completely independent in physical space. No matter in space or in use, each area should be in a completely separated state, and there is no direct connection of air.
Pressure control in the functional area of gene amplification laboratory
① The best pressure gradient is 10Pa and above
② Two control modes: system control and exhaust power change.
The system control means that the pressure control of four rooms should be started and stopped at the same time; the exhaust control means that the exhaust pipe should be branched, otherwise the air flow will flow backward when the laboratory is shut down, causing pollution.
2、 P2 laboratory decoration standard (laboratory decoration design)
P2 laboratory refers to the laboratory with biosafety protection reaching the second level. For the decoration design of biological secondary laboratory, the laboratory should be divided into three areas, namely: clean area, operation area, sterile area.
1. Clean area
The clean area of P2 laboratory usually consists of four parts: office, rest room, medium preparation room and reagent storage room. Note: it is forbidden to bring the bacterial test specimen into the clean area
2. Operation area
The operation area should be reflected in 7 aspects, namely: pollutant treatment, water source, temperature and humidity, ventilation, power supply, light and tidiness.
① Pollutant treatment: it is necessary to equip a disinfection tank for the treatment of contaminated articles. The remaining samples, plates and test tubes with bacteria should be placed in a centralized way, and then washed or discarded after toxic sterilization.
② Water source: the operation area should be equipped with a sink for handling specimens and washing hands of staff, and the two should not be mixed.
③ Temperature and humidity: in order to achieve the appropriate temperature and humidity, the operation area should be equipped with relevant air conditioning system.
④ Ventilation: in order to avoid air pollution, the operation area should be equipped with ventilation system.
⑤ Power supply: to be specific, the power supply should be stable and constant frequency. If the instrument has special requirements, it can be equipped with ups.
⑥ Light: the experimental project needs sufficient light for observation, so in addition to the conventional lighting in the operation area, the console lamp is also indispensable.
⑦ Tidiness: in order to make the operation area more tidy, in addition to the P2 laboratory decoration to do a good job in the daily use of the relevant precautions. For example, before entering the operation area, the staff should change into clean clothes, the specimens should be sent in and out through the transfer window, and disinfection work should be carried out at the beginning and end of the experimental project, and the cleaning work should be done every week.
3. Aseptic area
The sterile area should be equipped with air filter and air conditioner, and only aseptic operation can be carried out, and no bacteria operation can be carried out. Before operation, the staff should change into clean clothes, special masks, hats and shoes. In addition, the sterile area is a completely closed environment. The entrance and exit of the sterile area should pass through two doors, with a buffer room and a transfer window.
Believe to read this article aseptic room decoration After the introduction, we will have our own understanding of this aspect and solve the problems in the future.