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  • 无菌室work规程应该care什么?
    来源: 浏览:次 发布日期:2020-06-17
    (1)无菌室灭菌。每次使用上开启紫外线灯照射30min如该,或在使用上30min,对内外室用5百分比石炭酸喷雾。
    (1) Sterile room sterilization. Before each use, turn on the ultraviolet lamp for 30min or 30min before use, and use 5百分比 carbolic acid spray in the interior and exterior chambers.
    (2)用肥皂洗手下,把所需器材搬入外室;在外室换上已灭菌的work服、work帽和work鞋,戴好口罩,然下用2百分比煤酚皂液将手浸洗2分钟。
    (2) After washing hands with soap, move the required equipment into the outer room; put on sterilized work clothes, work caps and work shoes in the outer room, wear masks, and then wash hands with 2百分比 coal phenol soap for 2 minutes.
    (3)将各种需用物品搬进内室清点、就位,用5百分比石炭酸在work台面上方和操作员站位容量喷雾,返回外室,5~10min下再进内室work。
    (3) move all necessary articles into the inner room for inventory and position. Spray the 5百分比 carbolic acid on the working table and operator's space, return to the outer room, and then enter the inner room after 5 ~ 10min.
    (4)接种操作上,用70百分比酒精棉球擦手;进行无菌操作时,动作要轻缓,尽量减少空气波动和地上扬尘。
    无菌室装潢
    (4) Before inoculation operation, wipe hands with 70百分比 alcohol cotton ball; during aseptic operation, move gently to minimize air fluctuation and ground dust.
    (5)work中应care安全。如遇棉塞着火,用手紧握或用湿布包裹熄灭,切勿用嘴吹,以免扩大燃烧;如遇有菌培养物洒落或打碎有菌容器时,应用浸润5百分比石炭酸的抹布包裹下,并用浸润5百分比石炭酸的抹布擦拭台面或地上,用酒精棉球擦手下再继续操作。
    (5) Pay attention to safety in work. If the cotton plug catches fire, hold it tightly or wrap it with a wet cloth to extinguish it. Do not blow it with your mouth, so as not to expand the combustion. If the bacteria culture is spilled or smashed into the bacteria container, wrap it with a rag soaked with 5百分比 carbolic acid, wipe the table or ground with a rag soaked with 5百分比 carbolic acid, and then continue the operation after wiping your hands with an alcohol cotton ball.
    (6)work结束,立即将台面收拾干净,将不应在无菌室存放的物品和废弃物全部拿出无菌室下,对无菌室用5百分比石炭酸喷雾,或开紫外线灯照射30min。
    (6) at the end of the work, clean up the table immediately, and remove all the items and wastes that should not be stored in the asepsis room, then irradiate 30min with 5百分比 carbolic acid spray or ultraviolet lamp.
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