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  • 无菌室的策划装潢要按这七步走!
    来源: 浏览:次 发布日期:2019-05-31
      无菌室策划要旨:
      Design requirements of aseptic room:
      1. work使用室应短而平,面积仅为4米左右2、2.2-2.3米高,内部装潢应平整、光滑,无凹凸不平或棱角,墙面和屋顶应用不透水质料,便于擦洗和消毒。
      1. The studio should be short and flat, with an area of only about 4 meters and a height of 2,2-2.3 meters. The interior decoration should be smooth and smooth, without bumps or corners. The walls and roofs should be made of impermeable materials for easy scrubbing and disinfection.
      2. 房间内部照明面积大,应能从露天看到房间内部环境。
      2. The indoor lighting area is large, so we should see the indoor situation from the outdoor.
      3.为保证无菌室的清洁,应在无菌室周围设置缓冲走廊,并在走廊旁设置缓冲室,其面积可小于无菌室。
      3. In order to ensure the cleanliness of the sterile room, buffer corridors should be set up around the sterile room, and buffer rooms should be set up beside the corridor, whose area can be smaller than that of the sterile room.
      4. 无菌室、缓冲走廊和缓冲间均有日光灯和紫外线灯用于消毒空气,紫外线灯叶消毒work台以1米为宜,其电源开关均应位于露天。
      4. Fluorescent lamp and ultraviolet lamp are used to disinfect air in sterile room, buffer corridor and buffer room. Ultraviolet lamp leaf disinfection workbench is suitable for 1 meter, and its power switch should be located outdoors.
      5. 无菌室和缓冲室的出入口应设推拉门,门应与窗齐平,门缝应严密密封,两扇门应错开,避免空气豆泖污染。
      5. Push-pull doors should be installed at the entrances and exits of sterile rooms and buffer rooms. The doors should be level with windows, the door seams should be tightly sealed, and the two doors should be staggered to avoid air convection pollution.
    无菌室装潢
      6. 无菌室的使用和管理
      6. Use and management of sterile room
      1)无菌室应保持干净整洁,仅存放需要的检测设备,如酒精灯、酒精棉、火柴、镊子、接种针、接种环、玻璃铅笔等。
      1) The sterile room should be clean and tidy, and only store the most necessary testing equipment, such as alcohol lamp, alcohol cotton, matches, tweezers, inoculation needles, inoculation rings, glass pencils, etc.
      2)将房间内部检查用具、桌椅固定放置,不得随意移动。
      2) The indoor inspection equipment, tables and chairs shall be fixed and placed, and shall not be moved at will.
      3)每2-3周用2百分比石炭酸水溶液擦拭work台、门、窗、桌子、椅子和地上,然下用3百分比石炭酸水溶液喷雾对空气消毒,下对紫外线灯消毒半钟点。
      3) wipe the workbench, doors, windows, tables, chairs and ground with 2百分比 carbonic acid solution every 2-3 weeks, then disinfect the air with 3百分比 carbonic acid solution spray, and then disinfect the ultraviolet lamp for half an hour.
      4)定期检查房间内部空气无菌环境,细菌数量把握在10个以下。如果细菌不符合要旨,应立即彻底消毒。不育的方法测定无菌室:把三个共同肉汤的琼脂板和三个改良马丁培养基板块(直径9厘米),放在每一个无菌室的work地位,打开盖子,暴露了半个钟点,然下转化为底蕴。细菌总数置于37℃培养箱中培养48钟点。测量模具号应放置27℃培养箱培养5天。细菌、霉菌总数不得超过10个。
      4) Check the indoor air sterility regularly, and the number of bacteria should be under 10. If the bacteria do not meet the requirements, they should be thoroughly disinfected immediately. Sterility testing: three agar plates of common broth and three modified Martin culture substrates (9 cm in diameter) were placed in the working position of each sterile room, the lid was opened, exposed for half an hour, and then converted into culture. The total number of bacteria was incubated in 37 C incubator for 48 hours. Measuring die number should be placed in incubator at 27 C for 5 days. The total number of bacteria and moulds shall not exceed 10.
      5)无菌室消毒上,将一切物品放置于操作现场(待检测物品除外),打开紫外线灯消毒30分钟。当时间到了,关掉紫外线灯使用。
      5) Before sterilization in the sterile room, put all the items on the operation site (except the items to be tested) and turn on the ultraviolet lamp to disinfect for 30 minutes. When the time is up, turn off the ultraviolet lamp and use it.
      6)进入无菌室上,缓冲室必须更换已消毒的work服、work帽、work鞋。
      6) Before entering the sterile room, the buffer room must replace the disinfected work clothes, work caps and work shoes.
      7)严格按照无菌操作规程操作。为了保持环境的无菌状态,操作时应少说话、无噪音。
      7) Operate in strict accordance with aseptic operating procedures. In order to keep the environment aseptic, the operation should be less talk, no noise.
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